RNA
Part:BBa_K2457002:Design
Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano Group: iGEM17_Amazonas_Brazil (2017-07-09)
Optimized sgRNA targeting LacZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 2013.
Dang, Ying et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome biology, 2015.
Deltcheva, Elitza et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature, 2011.
Farboud, B., & Meyer, B. J. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics, 2015.
Jinek, Martin et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 2012.