RNA

Part:BBa_K2457002:Design

Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano   Group: iGEM17_Amazonas_Brazil   (2017-07-09)


Optimized sgRNA targeting LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 2013.

Dang, Ying et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome biology, 2015.

Deltcheva, Elitza et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature, 2011.

Farboud, B., & Meyer, B. J. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics, 2015.

Jinek, Martin et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 2012.